FastQCFastQC Report
Thu 2 Jun 2016
DRR015140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17377793
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492410.2833558898992524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427980.24627983541983725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254500.14645127836428942No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT181550.10447241488030154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA360350.058.441331
GTATCAA691600.042.4103781
GTGGTAT125300.034.006641
ATCAACG904500.032.3287283
TATCAAC913000.032.0104832
TCAACGC913800.031.9766654
CAACGCA956900.030.5791845
TGGTATC136250.029.8268642
AACGCAG986950.029.6571776
ACGCAGA1122900.026.0343887
GTACATG857100.025.8929841
CGCAGAG1147450.025.4773038
TACATGG856750.025.2255542
GCAGAGT1223200.023.6764359
ACATGGG891800.023.2794083
CATGGGT229800.020.450164
CAGAGTA1184600.020.30375310-11
GAGTACT724500.020.17535612-13
AGAGTAC1132100.019.97964110-11
AGTACTT717850.019.79188712-13
Produced by FastQC (version 0.11.5)