FastQCFastQC Report
Thu 2 Jun 2016
DRR015139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523642
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346190.27642917291950697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300140.23965871908507128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176070.14059009352071866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA259850.057.7501831
GTATCAA480850.040.5127071
GTGGTAT92500.033.1154331
ATCAACG621350.031.1385233
TGGTATC96750.030.8138332
TCAACGC629400.030.6727314
TATCAAC640750.030.324452
CAACGCA684600.028.125875
AACGCAG719750.026.8200326
GTACATG577500.025.985081
TACATGG571500.025.4693362
ACGCAGA793150.024.2524837
CGCAGAG793600.024.1452678
ACATGGG592100.023.0221023
GCAGAGT835350.022.6964389
CATGGGA261400.021.1185324
CATGGGG275250.020.0393564
CAGAGTA807350.020.00568610-11
GAGTACT502550.019.36322412-13
AGTACAT427500.019.1535992
Produced by FastQC (version 0.11.5)