FastQCFastQC Report
Thu 2 Jun 2016
DRR015139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523642
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387610.3095026191262893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348460.278241744693756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202480.16167820830394225No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT183590.14659473657902392No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG136050.10863453298968462TruSeq Adapter, Index 10 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280150.060.108691
GTATCAA526650.041.673371
GTGGTAT91150.035.7102551
TGGTATC95600.033.0830572
ATCAACG691500.031.6337283
TCAACGC703650.031.1111324
TATCAAC710000.030.8614752
CAACGCA746000.029.3327755
AACGCAG781300.028.0790356
GTACATG588700.025.1478041
ACGCAGA872100.025.1296657
CGCAGAG872750.025.0799798
TACATGG586950.024.4301412
GCAGAGT919450.023.524339
ACATGGG601500.022.7671663
CAGAGTA890450.020.67433410-11
CATGGGA264450.020.3339044
CATGGGG272450.020.1375854
GAGTACT570000.020.0454412-13
AGAGTAC834700.019.921610-11
Produced by FastQC (version 0.11.5)