FastQCFastQC Report
Thu 2 Jun 2016
DRR015138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11847709
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254650.21493606907462023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220140.1858080747931942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125650.10605425909768716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226600.055.217161
GTATCAA413350.040.940271
GTGGTAT85700.033.5656851
ATCAACG525500.031.8723553
TCAACGC529200.031.491384
TATCAAC541700.031.096812
TGGTATC98000.028.423722
CAACGCA587750.028.3996735
AACGCAG614500.027.2213786
GTACATG578400.025.9605121
TACATGG573750.025.5043262
ACGCAGA660150.025.2786147
CGCAGAG665150.025.059758
ACATGGG604800.023.2834633
GCAGAGT711750.022.9753849
CATGGGG302200.020.9622524
CATGGGA283700.020.5325954
CAGAGTA687300.020.14187610-11
GAGTACT389800.019.99370412-13
GTACTTT391900.019.62987114-15
Produced by FastQC (version 0.11.5)