FastQCFastQC Report
Thu 2 Jun 2016
DRR015138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11847709
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271740.22936079878396742No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT250650.21155988892029676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246010.20764351994128147No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT149030.12578803209970804RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136910.11555820623210783No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG132240.11161651590193514RNA PCR Primer, Index 31 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230450.054.560921
GTATCAA420150.040.523031
GTGGTAT85150.034.4299131
ATCAACG542550.031.2365153
TCAACGC547850.030.9481934
TATCAAC559500.030.3053782
CAACGCA594650.028.5507725
TGGTATC101800.028.2965092
AACGCAG623050.027.3111536
GTACATG581800.025.2029691
TACATGG576750.024.8384252
ACGCAGA691350.024.5476477
CGCAGAG699600.024.1869588
ACATGGG602550.023.0143093
GCAGAGT742700.022.5138729
CATGGGA280350.020.6244584
CATGGGG301450.020.1768674
CAGAGTA715000.019.61129210-11
GAGTACT412750.019.0683112-13
AGAGTAC691400.018.9352310-11
Produced by FastQC (version 0.11.5)