FastQCFastQC Report
Thu 2 Jun 2016
DRR015137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365478
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391300.21306279096030062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335450.18265247438699936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198750.1082193450124195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA326750.057.780071
GTATCAA639350.041.6957171
GTGGTAT106950.035.875811
TGGTATC113850.032.7415052
ATCAACG824950.032.004513
TCAACGC825000.031.9060884
TATCAAC857800.030.997262
CAACGCA873650.030.1442575
AACGCAG914400.028.8691446
GTACATG956950.026.734941
TACATGG938850.026.2657972
ACGCAGA1030650.025.5809787
CGCAGAG1052700.025.0045578
ACATGGG978150.024.0558683
GCAGAGT1112750.023.334259
CATGGGA403200.022.3369834
CATGGGG471750.020.817994
GTACTTT574200.019.95643214-15
CAGAGTA1096600.019.69876110-11
GCTCTAG51150.019.4873751
Produced by FastQC (version 0.11.5)