FastQCFastQC Report
Thu 2 Jun 2016
DRR015137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365478
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435230.23698266933210232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389580.21212625121981582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226170.12314953087526499No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA347850.058.4348221
GTATCAA680700.042.0665741
GTGGTAT108750.037.717061
TGGTATC119600.033.1807942
ATCAACG875500.032.427363
TCAACGC880700.032.2331774
TATCAAC906450.031.3086052
CAACGCA922150.030.7448525
AACGCAG963250.029.4895256
GTACATG949800.026.308941
ACGCAGA1093150.025.9817357
TACATGG931750.025.916082
CGCAGAG1116300.025.4144698
GCAGAGT1180350.023.8543289
ACATGGG974450.023.8165553
CATGGGA391300.021.36894
CATGGGG477200.021.0973284
GCTCTAG47400.020.9372881
CAGAGTA1168950.020.01926810-11
GTACTTT655000.019.84658614-15
Produced by FastQC (version 0.11.5)