FastQCFastQC Report
Thu 2 Jun 2016
DRR015136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14648887
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375410.25627202940400867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314680.2148149548836031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191030.13040581171798238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296450.058.8970871
GTATCAA583300.042.9724161
GTGGTAT112550.033.363771
ATCAACG748350.033.091423
TGGTATC108300.032.739442
TCAACGC759600.032.4970174
TATCAAC775400.032.126392
CAACGCA815700.030.2446655
AACGCAG853300.028.9948166
GTACATG836550.027.204751
TACATGG827750.026.707372
CGCAGAG952500.025.8528358
ACGCAGA955650.025.8512827
CATGGGT292700.025.748174
ACATGGG833350.025.1776333
GCAGAGT1019300.023.8786729
ATGGGTA134950.020.267565
CATGGGG335950.020.076244
CAGAGTA1002550.020.00464610-11
GAGTACA635750.019.8889831
Produced by FastQC (version 0.11.5)