FastQCFastQC Report
Thu 2 Jun 2016
DRR015136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14648887
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416550.28435607428741855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363730.24829872740502404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216790.14799076544177042No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT169530.11572892875752268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA316800.059.80821
GTATCAA620350.042.313771
GTGGTAT113100.033.4891851
TGGTATC111100.033.342522
ATCAACG805800.032.5448653
TCAACGC818500.032.036724
TATCAAC826100.031.6865042
CAACGCA867200.030.2428555
AACGCAG899800.029.2234526
GTACATG846800.026.8425981
TACATGG840700.026.4484672
ACGCAGA1022200.025.7069117
CGCAGAG1019550.025.6983248
CATGGGT302350.025.32314
ACATGGG842050.025.081473
GCAGAGT1095450.023.7476069
CATGGGG337750.020.5677344
ATGGGTA136250.020.4675885
GAGTACT634050.020.37046412-13
CAGAGTA1078850.020.09763310-11
Produced by FastQC (version 0.11.5)