FastQCFastQC Report
Thu 2 Jun 2016
DRR015135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050868
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352460.35067618040551324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298360.2968499835039123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179930.1790193642976905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236750.062.3264581
GTATCAA481600.043.0856361
GTGGTAT75400.040.564611
TGGTATC73750.040.4222
ATCAACG622500.033.0680433
TCAACGC633400.032.4603084
TATCAAC667850.031.0282522
CAACGCA689150.029.8403875
AACGCAG717850.028.7091736
CGCAGAG776250.026.4182858
GTACATG606800.026.3125821
ACGCAGA784350.026.217417
TACATGG594000.026.020752
GCAGAGT821250.024.6920249
ACATGGG603150.024.2743173
CATGGGA299100.022.578864
CATGGGG219100.021.3310664
CAGAGTA812800.020.77293810-11
GTCGGGA39950.020.6205562
GTACTTT479950.019.8815714-15
Produced by FastQC (version 0.11.5)