FastQCFastQC Report
Thu 2 Jun 2016
DRR015135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050868
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379190.37727089839404915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343010.34127400737926317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196750.19575423734547107No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT128210.1275611220841822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA259850.062.7286031
GTGGTAT78250.042.5223271
GTATCAA514900.042.0615771
TGGTATC78650.041.6586652
ATCAACG679450.031.7931863
TCAACGC693150.031.1882574
TATCAAC717550.030.1050012
CAACGCA738200.029.247965
AACGCAG773100.027.9600096
GTACATG598750.025.3532891
CGCAGAG852400.025.3001488
ACGCAGA861100.025.0608777
TACATGG585950.024.9684732
GCAGAGT901650.023.6055289
ACATGGG601350.023.0226553
CATGGGA301050.021.8963474
CAGAGTA888950.020.4304610-11
CATGGGG219300.019.9769674
GTCGGGA38550.019.7134972
GTACTTT564050.019.7066914-15
Produced by FastQC (version 0.11.5)