FastQCFastQC Report
Thu 2 Jun 2016
DRR015134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19596521
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510870.26069423240992623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438950.2239938405393488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251220.12819622421755372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA384800.057.874541
GTATCAA774900.043.135461
GTGGTAT142000.034.3281
ATCAACG989500.033.4575233
TCAACGC996250.033.1173824
TATCAAC1010050.032.8946382
TGGTATC144950.032.2161982
CAACGCA1032800.031.962525
AACGCAG1084600.030.443236
GTACATG1280950.029.5765341
TACATGG1263100.029.2196332
ACATGGG1298100.026.7763213
ACGCAGA1283650.025.6891847
CGCAGAG1293250.025.475568
CATGGGT244800.025.375094
CATGGGA527350.023.8175164
GCAGAGT1380350.023.5950269
CATGGGG550100.022.997974
AGTACAT897350.022.3727992
GAGTACA969600.021.9168761
Produced by FastQC (version 0.11.5)