FastQCFastQC Report
Thu 2 Jun 2016
DRR015134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19596521
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT568290.28999535172595176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508970.25972467255795045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285200.1455360367281519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA421100.059.2667771
GTATCAA837450.042.6255571
GTGGTAT143500.036.262011
TGGTATC150900.033.5660672
ATCAACG1081150.032.8780983
TCAACGC1090450.032.591444
TATCAAC1098050.032.3685652
CAACGCA1124950.031.5919255
AACGCAG1183700.030.1124866
GTACATG1284000.028.1167851
TACATGG1261900.027.7581832
ACATGGG1294200.025.7678013
ACGCAGA1398200.025.4740817
CGCAGAG1408700.025.2426788
CATGGGT246500.024.4722374
GCAGAGT1504900.023.5051789
CATGGGA521600.022.97344
CATGGGG552150.021.8670864
AGTACAT911450.021.729542
GAGTACA985850.021.1177641
Produced by FastQC (version 0.11.5)