FastQCFastQC Report
Thu 2 Jun 2016
DRR015133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872105
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197240.22231477197350571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171210.19297562416134617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.11036839622614926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158250.054.443031
GTATCAA289650.039.801281
GTGGTAT64250.032.018481
TGGTATC65100.030.3418242
ATCAACG376350.030.3171233
TATCAAC379900.030.2619552
TCAACGC376650.030.2056664
CAACGCA431650.026.3349425
GTACATG394150.025.5653341
AACGCAG449000.025.3363066
TACATGG388400.025.264022
ACGCAGA468200.024.2838947
CGCAGAG478750.023.7851628
ACATGGG405550.023.1291243
CATGGGT105350.023.0671044
GCAGAGT502850.022.3624829
CATGGGG209700.020.9852664
GTACTTT277500.019.63536314-15
CAGAGTA479000.019.6192110-11
AGTACAT292000.019.0623552
Produced by FastQC (version 0.11.5)