FastQCFastQC Report
Sun 19 Mar 2023
SRR6026846_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026846_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217705443
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18681020.8580869519187906No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC8766440.4026743603282349No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5837490.2681370717956739No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA5012880.2302597459632647No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACT4558560.2093911818272729No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT4515380.20740776793531984No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4423780.2032002479607274No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT3892570.17879984746178348No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGAT2412930.11083461978486225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGT24503750.0138.564222
CACTGTG40892850.0138.492611
ACTGTGG19826600.0137.610032
GTGTGAC8521850.0136.144235
CTGTGTG16168000.0136.141243
TGTGACT6486700.0135.581136
GACTAGT2898950.0134.259481
TGTGTGC8336500.0133.94064
CTGTGTC9719750.0133.69933
CAAGCTA4864050.0133.636522
CTGTGGC9020950.0133.333183
AGAGTCA3194650.0133.125461
ACAAGCT5418700.0132.525421
TGTGTGA8617400.0132.261124
GTGACTC3400450.0132.013697
ACTAGTA2699800.0131.788532
TGTGCCT5995600.0131.764916
GTGTGCC8709300.0131.722665
TGTGTCA3904450.0130.168614
CCGAAGT3782750.0129.86211
Produced by FastQC (version 0.11.5)