FastQCFastQC Report
Mon 6 Jun 2016
ERR1378509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775083
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTAAATCTCGTATGCCGTC51730.6674123932533677Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTAAATCTCGTATGCC50570.6524462541431046Illumina Paired End PCR Primer 2 (97% over 37bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT18050.23287828529331694Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA207.031564E-445.0003932
TATGCCG5850.037.31523543
ATGCCGT6000.036.38235544
ACGCTAG250.00210659736.0003131
TTACGAC250.002107931535.99566722
TGCCGTC6150.035.4949845
GGCGGAC300.005141149730.00606744
CGGAAGA9800.025.5013641
AACGCTA450.001226132625.00021630
ATCGGAA3550.024.08471527
GATCGGA3600.023.74867226
AGATCGG3900.022.4972925
TCGGAAG4000.021.93768928
GTATGCC11300.021.70792845
CGAACTA11100.021.48667132
CCGAGAT4150.021.14203322
CGTATGC11450.021.03045344
ACCGAAC11350.021.01339730
TCTCGTA11400.020.92663441
GACCGAA11450.020.82987429
Produced by FastQC (version 0.11.5)