FastQCFastQC Report
Mon 6 Jun 2016
ERR1391250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484111
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA36350.7508608562912225No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA29000.5990361714565461No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATGTATCTCGTATGCCGTC10310.21296768716265482Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATGTATCTCGTATGCC9540.1970622439894983Illumina Paired End PCR Primer 2 (96% over 33bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA5780.11939410589720126No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT5180.10700025407396237Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT3800.043.21713615
GACGTCA3800.043.21713622
AAGCCGA3800.043.21713614
TAAGCCG3800.043.21713613
CCGATGA3850.042.65587617
CGATGAC3850.042.65587618
GATGACG3950.042.1455119
TGACGTC3900.042.10900521
ATGACGT3900.042.10900520
ACGTCAT3900.042.10900523
GCGGTCT3350.041.63548336
GGCGGTC3350.041.63548335
GCCGATG3950.041.57597716
CGTCATA3900.041.5321724
AGGCGGT3400.041.02319734
TTCGGTA3400.041.02319722
TCGGTAT3400.041.02319723
TCCTCGG3100.040.66840711
CGGTATT3500.040.49386624
AATTCGG3400.040.36153420