FastQCFastQC Report
Mon 6 Jun 2016
ERR1391166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452815
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA33190.7329704183827832No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA23390.5165464924969358No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10340.228349325883639Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCAGTATCTCGTATGCCGTC9040.19964002959265925Illumina Paired End PCR Primer 2 (96% over 33bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA4960.10953700738712277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCAT3650.044.3830223
TAAGCCG3750.043.7994613
AGCCGAT3800.043.22315615
CCGATGA3750.043.1994717
GACGTCA3750.043.1994722
TGACGTC3750.043.1994721
ATGACGT3750.043.1994720
ATCGGAA1250.043.1994727
AAGCCGA3850.042.66181614
ATTCGGT2850.042.63105821
GCCGATG3800.042.63105816
AATTCGG2850.042.63105820
GATGACG3850.042.07740819
CGATGAC3850.042.07740818
CGTCATA3850.042.07740824
TTCGGTA2900.041.89603422
TTCCTCG2500.041.3994910
CGGTATT2950.041.18593624
TCGGTAT2950.041.18593623
TCGGGGA2900.041.12018214