FastQCFastQC Report
Mon 6 Jun 2016
ERR1391281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339408
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAAATA42241.2445198698911044No Hit
TCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA30090.8865436289068024No Hit
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCCGTC24680.7271484467072079Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGAGTATCTCGTATGCC20160.5939753924480272Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11630.342655447131476Illumina Paired End PCR Primer 2 (96% over 30bp)
TCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATCCAA6260.18443878753594495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG4200.044.4622516
CCGATGA4250.043.93916317
TAAGCCG4250.043.93916313
CGTCATA4250.043.93916324
GACGTCA4300.043.42824622
AGCCGAT4350.042.9290615
ACGTCAT4350.042.9290623
GATGACG4400.042.44123519
CGATGAC4400.042.44123518
TGACGTC4550.041.5365621
ATGACGT4500.041.49809620
CGGTATT4600.041.08507524
AAGCCGA4550.041.04207614
CTCGGGG3950.041.0107813
ATTCGGT4700.040.6896321
GCGGTCT4650.040.64329536
TTCGGTA4650.040.64329522
TCGGGGA4700.040.21092614
GGCGGTC4700.040.21092635
AATTCGG4700.040.21092620