FastQCFastQC Report
Mon 6 Jun 2016
ERR1378927.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378927.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579410
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCATCTCGTATGCC121862.103173918296198Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC64971.1213130598367305Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT13910.2400717971729863Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGAAACCATCTCGTATGCCGTC6000.10355361488410623Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAC16050.036.02762232
CCGGAAA16000.035.99958831
GTATGCC15400.035.94114345
GACCGGA16150.035.66522629
TCTCGTA15600.035.6245941
CTCGTAT15600.035.48036242
ATCTCGT15750.035.42816540
GAGACCG16350.035.2289527
ACCGGAA16400.035.12154830
AGACCGG16400.035.12154828
CGTATGC15850.035.06268744
CATCTCG16000.034.7339839
TCGTATG15950.034.70179443
AGATCGG10600.034.38639517
GATCGGA10850.033.80145618
AACCATC16750.033.7160336
GAGATCG10750.033.69728516
CGAGACC17350.033.5875126
ACCATCT16950.033.45094337
TCGGAAG10850.033.38671520