FastQCFastQC Report
Mon 6 Jun 2016
ERR1378970.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378970.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751217
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT66770.8888244009387434Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCCGTC32590.43382937287095474Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC16180.21538383716023465Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC8540.11368219835280619Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3750.036.6052543
GATCGGA8500.036.52492526
ATCGGAA8500.036.52492527
TCGGAAG8800.036.30236428
ATGCCGT3850.035.65446544
TGCCGTC3900.035.1973645
AGATCGG8850.034.82623325
CCGAGAT9850.031.06217822
GAGATCG10050.030.44402324
CGAGATC10200.029.99631923
AATGCCG15100.029.94665518
ATGCCGA15150.029.8478219
TGCCGAG15200.029.74963820
CGGAAGA11000.029.0418929
GCCGAGA15800.028.19274121
GAATGCC16300.028.01989617
GGAATGC17350.026.32416716
AGGAATG18200.025.09474215
GACCGGT5450.024.76760126
ACCGGTA5500.024.5424427