FastQCFastQC Report
Mon 6 Jun 2016
ERR1379127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675046
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCCGTC21540.3190893657617407Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC20790.30797901180067727Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.036.381943
ATGCCGT2950.028.21950344
CGGAAGA4400.026.6315571
TGCCGTC3300.025.22652645
CTCGTAT4750.023.6835142
TCGTATG4700.023.4567543
TCTCGTA4800.023.43680441
ACCGGTG4850.023.19175130
GACCGGT4850.023.19175129
CGTATGC4800.022.9680744
CCGGTGT5100.022.05980331
AGACCGG5100.022.05490128
GTATGCC5000.022.04934745
ATCGGAA1459.094947E-1221.71866827
CGGTGTG5450.021.46884532
ATCTCGT5250.021.42793540
AATCTCG5250.021.42793539
GAAGAGC5500.021.289453
GATCGGA1501.4551915E-1120.9947126
CCGAGAC5500.020.44939425