FastQCFastQC Report
Mon 6 Jun 2016
ERR1379139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977692
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCCGTC52560.5375926160795015Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACAAATCTCGTATGCC51420.5259325022604255Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT15600.15955945226103926Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9810.10033834786415353Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6150.038.04523543
ATGCCGT6850.034.4858444
TGCCGTC7300.032.6681945
CGGAAGA10850.026.156031
CGATACA11600.023.27488132
CCGATAC11750.022.78627631
CGTATGC11800.022.68856444
CTCGTAT11800.022.68856442
TCTCGTA11900.022.49790241
ACCGATA11950.022.4037730
TCGTATG12000.022.31042143
GACCGAT12050.022.21784629
GTATGCC12050.022.21784645
GAAGAGC12900.021.991573
AGACCGA12250.021.85510628
GAGACCG12550.021.50973927
AATCTCG12650.021.16403439
ATCTCGT13000.020.76729440
GATACAA13700.020.03565433
GGAAGAG14650.019.8286972