FastQCFastQC Report
Mon 6 Jun 2016
ERR1378831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663737
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT29750.44821970147814566Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAATAATCTCGTATGCC28900.43541342429305585Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAATAATCTCGTATGCCGTC22690.3418522697996345Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3150.036.4224743
ATGCCGT3150.036.4224744
ATCGGAA4750.032.67873827
TCGGAAG5000.032.39457728
AGATCGG4900.031.21926125
GATCGGA4900.031.21926126
TGCCGTC3700.030.40031445
CGAGATC5950.026.0880723
CCGAGAT6150.024.87388222
GAGATCG6250.024.47590324
CGTATGC6250.024.11596344
TCGTATG6250.024.11596343
AATGCCG11350.023.98276918
CTCGTAT6350.023.73618342
ACCGGTA6300.023.56748230
CCGGTAA6350.023.38367331
GTATGCC6450.023.3681845
TCTCGTA6600.023.17793741
TGCCGAG12000.023.05863820
ATCTCGT6800.022.8270640