FastQCFastQC Report
Mon 6 Jun 2016
ERR1378869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256154
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT37160.295823601246344Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCCGTC26090.20769746384599339Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCC21460.17083892580049898Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3200.038.66653443
ATGCCGT3650.034.51578544
GATCGGA5050.033.4085726
TGCCGTC3900.031.72638945
ATCGGAA5550.030.39878827
TCGGAAG5750.030.12507828
AGATCGG5600.029.72567225
ATGCCGA10650.028.09247819
AATGCCG10650.027.88125618
TGCCGAG10950.027.11738620
CGTATGC4900.025.25161641
CTCGTAT4950.024.99655239
CCGAACC5100.024.26039128
TCTCGTA5100.024.25942638
CCGAGAT7100.024.07926622
GAATGCC12850.023.80804817
TATCTCG5200.023.79668636
TCGTATG5250.023.56817640
GCCGAGA12550.023.48094621
ACCGAAC5300.023.34397727