FastQCFastQC Report
Mon 6 Jun 2016
ERR1378811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955501
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCTAATCTCGTATGCCGTC27280.28550467241792526Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCTAATCTCGTATGCC17450.1826267057805277Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT11080.11596010888528635Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.043.49661343
ATGCCGT3400.037.71764844
TGCCGTC3450.037.17101745
CGTATGC5250.024.85520641
CGTACCT5350.024.81244929
TCTCGTA5300.024.62201138
CTCGTAT5350.024.391939
CCGTACC5450.024.35717628
ACCGTAC5450.024.35717627
TCGTATG5400.024.16478340
GACCGTA5550.023.91830626
AATCTCG5800.023.66318936
ATCTCGT5700.023.2888837
GAGACCG5700.023.2888824
AGACCGT5700.023.2888825
GTACCTA6200.021.77363630
GTATGCC6100.021.39177742
GCGGTTC9200.020.5504825
TGCCGAG9200.020.05278820
GAGCGGT9550.020.0330053