FastQCFastQC Report
Mon 6 Jun 2016
ERR1378863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975323
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT56180.5760143050045985Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCCGTC45690.4684601921619812Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCC40670.41699006380450376Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT6000.039.36849644
TATGCCG6200.038.46138843
TGCCGTC6250.037.79375545
ATCGGAA7900.033.03082727
AGATCGG8050.032.97591825
TCGGAAG8500.032.28878428
GATCGGA8100.032.2152526
CCGAGAT9400.028.24001322
CGAGATC9350.028.1504323
GAGATCG9700.027.36661324
ATGCCGA19450.027.06493619
AATGCCG19450.027.06354718
GTCGGAA507.167861E-526.99415627
TGCCGAG19600.026.85780720
GAATGCC20550.025.61489117
GCCGAGA20500.024.91051921
CTTCGTA851.8932406E-723.8232578
GGAATGC22150.023.76460616
GACCGTC10500.023.35208726
TCTCGTA10350.023.25583538