FastQCFastQC Report
Mon 6 Jun 2016
ERR1378894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880139
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCC90461.027792201004614Illumina Paired End PCR Primer 2 (96% over 33bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC42310.4807195227117535Illumina Paired End PCR Primer 2 (100% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT9400.10680131206548056Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13450.038.31106642
CCGGATG13600.038.0496531
CGGATGC13600.038.0496532
ATCTCGT13650.037.9102740
TCTCGTA13650.037.9102741
CGTATGC13700.037.77620344
ACCGGAT13700.037.7719130
TATCTCG13750.037.6345639
GACCGGA13900.037.5521629
TCGTATG13800.037.5024643
GTATGCC13850.037.04214545
AGACCGG14150.036.88868728
GAGACCG14200.036.758827
CGAGACC15250.034.52293426
GCTATCT15000.034.49834437
GGATGCT15200.034.0444233
GATGCTA15250.033.932834
AGATCGG7450.033.8238817
TGCTATC15550.033.13345736
GATCGGA7650.032.93959418