FastQCFastQC Report
Mon 6 Jun 2016
ERR1378989.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378989.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055389
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT53030.5024687579650726Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCCGTC27110.25687211066251403Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACTAGATCTCGTATGCC15160.1436437180982557Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA7550.033.673927
GATCGGA7700.033.01791426
AGATCGG7850.032.6736125
TATGCCG3100.032.66283843
TCGGAAG8000.032.3422228
ATGCCGT3500.028.92994344
ATGCCGA13050.027.93103419
AATGCCG13100.027.65267218
TGCCGAG13400.027.20020320
CCGAGAT9700.026.90594122
CGGAAGA9650.026.812229
GAGATCG9650.026.812224
TGCCGTC3900.025.96276945
GAATGCC14150.025.91872817
CGGACTA4150.024.93857829
GCCGAGA14600.024.65636621
CGAGATC10400.024.44595723
ACCGGAC4350.024.30919327
CCGGACT4300.024.06862828
GACCGGA4350.023.79197726