FastQCFastQC Report
Mon 6 Jun 2016
ERR1378976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748547
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT68560.9159077519514472Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCCGTC41850.5590831303845984Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC12930.17273464458477555Illumina Paired End PCR Primer 2 (97% over 35bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC9140.1221032213074129Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5750.039.5288443
ATGCCGT5750.039.5288444
TGCCGTC5900.038.5238745
CGATACT6450.035.2271229
ACCGATA6450.034.87833427
CCGATAC6500.034.6100428
GACCGAT6500.034.6100426
CGTATGC6650.034.5151741
AGACCGA6650.033.8293625
GATCGGA8000.033.7447926
CTCGTAT6750.033.67046439
TCTCGTA6850.033.1789338
TCGTATG6850.033.1789340
GAGACCG6850.032.84164424
AATGCCG15300.032.78905518
TCGGAAG8400.032.67352728
ATCTCGT7050.032.55686637
AGATCGG8350.032.33033825
ATGCCGA15600.032.01428619
GTATGCC7200.031.8807342