FastQCFastQC Report
Mon 6 Jun 2016
ERR1378792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766138
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTAGTATCTCGTATGCCGTC16720.21823744547326984Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTAGTATCTCGTATGCC14790.19304616139651082Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2100.038.56902343
ATGCCGT2200.036.81588744
TGCCGTC2350.034.46593545
TCTCGTA3350.023.50599738
CCGCTTA3450.023.47679728
AGATCGG1750.023.14141325
CGCTTAG3500.023.14141329
CGTATGC3600.022.49859641
GATCGGA1800.022.49859626
ACCGCTT3600.022.49859627
GACCGCT3500.022.49859626
GCGCCCG702.7027756E-522.49859612
AGACCGC3500.022.49859625
TATCTCG3550.022.18171536
TCGTATG3800.021.90652840
CTCGTAT3700.021.89052839
GAGACCG3600.021.87363624
ATCGGAA2050.020.85235827
ATCTCGT3800.020.72239137
AATGCCG5950.020.41889418