SCPortalen


Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing.


According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcγRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor GATA-1 generated mast cells, eosinophils, megakaryocytes and erythroid cells, and a pathway lacking expression of that gene generated monocytes, neutrophils and lymphocytes. These results identify an early hematopoietic-lineage bifurcation that separates the myeloid lineages before their segregation from other hematopoietic-lineage potential.

GSE77029 Samples



Cell Metadata and BAM Files

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BAM Files Run Accession Number Sample Accession Number Organism Organ/Tissue Dev. Stage Cell Type Sequencer Protocol Assay Type Library Layout Ontology Term Cell Cycle Phase
SRR3110179 SAMN04431025 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110180 SAMN04431026 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110181 SAMN04431027 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110182 SAMN04431028 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110183 SAMN04431029 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110184 SAMN04431030 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110185 SAMN04431031 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110186 SAMN04431032 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110187 SAMN04431033 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110188 SAMN04431034 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110189 SAMN04431035 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110190 SAMN04431036 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110191 SAMN04431037 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110192 SAMN04431038 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110193 SAMN04431039 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110194 SAMN04431040 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110195 SAMN04431041 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110196 SAMN04431042 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110197 SAMN04431043 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110198 SAMN04431044 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110199 SAMN04431045 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110200 SAMN04431046 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110201 SAMN04431047 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110202 SAMN04431048 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110203 SAMN04431049 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110204 SAMN04431050 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110205 SAMN04431051 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110206 SAMN04431052 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110207 SAMN04431053 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110208 SAMN04431059 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110209 SAMN04431060 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110210 SAMN04431061 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110211 SAMN04431062 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110212 SAMN04431063 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110213 SAMN04431064 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110214 SAMN04431065 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110215 SAMN04431066 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110216 SAMN04431067 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110217 SAMN04431068 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110218 SAMN04431069 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110219 SAMN04431070 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110220 SAMN04431071 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110221 SAMN04431072 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110222 SAMN04431073 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110223 SAMN04431074 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110224 SAMN04431075 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110225 SAMN04431076 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110226 SAMN04431077 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110227 SAMN04431078 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110228 SAMN04431079 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110229 SAMN04431080 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110230 SAMN04431081 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110231 SAMN04431084 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] S
SRR3110232 SAMN04431085 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110233 SAMN04431086 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110234 SAMN04431087 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110235 SAMN04431088 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110236 SAMN04431054 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110237 SAMN04431055 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110238 SAMN04431056 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2
SRR3110239 SAMN04431082 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1
SRR3110240 SAMN04431083 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G2.M
SRR3110241 SAMN04431057 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] G1.S
SRR3110242 SAMN04431058 Mus musculus Bone Marrow Adult Bone marrow Illumina HiSeq 2000 SMARTer RNA-Seq Single-End UBERON:0002371 [bone marrow] M.G1

FASTQC Report



FASTQC File ID Cell Accession Number FASTQC Report Link No. Sequence Tags
SRR3110179_1 SRR3110179 Preview 11368075
SRR3110180_1 SRR3110180 Preview 6836112
SRR3110181_1 SRR3110181 Preview 12493075
SRR3110182_1 SRR3110182 Preview 11041770
SRR3110183_1 SRR3110183 Preview 10436348
SRR3110184_1 SRR3110184 Preview 10159752
SRR3110185_1 SRR3110185 Preview 9835326
SRR3110186_1 SRR3110186 Preview 19286174
SRR3110187_1 SRR3110187 Preview 10433542
SRR3110188_1 SRR3110188 Preview 6354097
SRR3110189_1 SRR3110189 Preview 10707733
SRR3110190_1 SRR3110190 Preview 8388111
SRR3110191_1 SRR3110191 Preview 7978710
SRR3110192_1 SRR3110192 Preview 11062341
SRR3110193_1 SRR3110193 Preview 1710618
SRR3110194_1 SRR3110194 Preview 3830705
SRR3110195_1 SRR3110195 Preview 9925975
SRR3110196_1 SRR3110196 Preview 9052874
SRR3110197_1 SRR3110197 Preview 3592157
SRR3110198_1 SRR3110198 Preview 3033035
SRR3110199_1 SRR3110199 Preview 1237909
SRR3110200_1 SRR3110200 Preview 6867023
SRR3110201_1 SRR3110201 Preview 1064672
SRR3110202_1 SRR3110202 Preview 5614096
SRR3110203_1 SRR3110203 Preview 1046836
SRR3110204_1 SRR3110204 Preview 2267101
SRR3110205_1 SRR3110205 Preview 4929473
SRR3110206_1 SRR3110206 Preview 7054441
SRR3110207_1 SRR3110207 Preview 3551087
SRR3110208_1 SRR3110208 Preview 9972940
SRR3110209_1 SRR3110209 Preview 3267948
SRR3110210_1 SRR3110210 Preview 4549640
SRR3110211_1 SRR3110211 Preview 10439194
SRR3110212_1 SRR3110212 Preview 7070529
SRR3110213_1 SRR3110213 Preview 11150464
SRR3110214_1 SRR3110214 Preview 8850555
SRR3110215_1 SRR3110215 Preview 8305591
SRR3110216_1 SRR3110216 Preview 7519059
SRR3110217_1 SRR3110217 Preview 9537724
SRR3110218_1 SRR3110218 Preview 8223598
SRR3110219_1 SRR3110219 Preview 7629249
SRR3110220_1 SRR3110220 Preview 7105584
SRR3110221_1 SRR3110221 Preview 9116369
SRR3110222_1 SRR3110222 Preview 6388286
SRR3110223_1 SRR3110223 Preview 8166022
SRR3110224_1 SRR3110224 Preview 10845999
SRR3110225_1 SRR3110225 Preview 5393763
SRR3110226_1 SRR3110226 Preview 7432431
SRR3110227_1 SRR3110227 Preview 5129884
SRR3110228_1 SRR3110228 Preview 10269997
SRR3110229_1 SRR3110229 Preview 9919060
SRR3110230_1 SRR3110230 Preview 10263425
SRR3110231_1 SRR3110231 Preview 6763225
SRR3110232_1 SRR3110232 Preview 8726071
SRR3110233_1 SRR3110233 Preview 7016179
SRR3110234_1 SRR3110234 Preview 7319490
SRR3110235_1 SRR3110235 Preview 4890948
SRR3110236_1 SRR3110236 Preview 11964978
SRR3110237_1 SRR3110237 Preview 7049877
SRR3110238_1 SRR3110238 Preview 949675
SRR3110239_1 SRR3110239 Preview 8245275
SRR3110240_1 SRR3110240 Preview 5807939
SRR3110241_1 SRR3110241 Preview 4597415
SRR3110242_1 SRR3110242 Preview 16623538

Organism : Mus musculus

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File Size : 137M

Mouse Cell Data

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tSNE image.
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PCA image.
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Gene Body Coverage image.

Data Resources


Organism Run Metadata Link Data Repository Link Pubmed Record Link Author Provided Files DAVID Annotation Chart Link DAVID Annotation Table Link Gene Body Coverage Matrix Link PCA Matrix Link Krona Plot Img Link Unmapped Reads Link Expression Correlation
Mus musculus