Human B cells from a healthy donor post-flu vaccination (v2)

Human B cells isolated from a healthy female donor 14 days post-influenza vaccination were obtained by 10x Genomics from Discovery Life Sciences. Gene expression and BCR Amplified libraries were generated from 16,400 cells (85% viable, expected recovery 8,500 cells) as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A) and sequenced on an Illumina NovaSeq 6000. These libraries were analyzed using the Cell Ranger multi pipeline, which combines Gene Expression, Cell Surface Protein/Immune Receptor Mapping, and V(D)J Amplified libraries from the same sample. Cell Ranger multi enables consistent cell calling between gene expression and V(D)J libraries.

B_postvax_5gex_B Samples

BAM Files Run Accession Number Sample Accession Number Organism Organ/Tissue Dev. Stage Cell Type Sequencer Protocol Assay Type Library Layout Ontology Term Cell Cycle Phase


FASTQC File ID Cell Accession Number FASTQC Report Link No. Sequence Tags

Data Resources

Organism Run Metadata Link Data Repository Link Pubmed Record Link Author Provided Files DAVID Annotation Chart Link DAVID Annotation Table Link Gene Body Coverage Matrix Link PCA Matrix Link Krona Plot Img Link Unmapped Reads Link Expression Correlation